Part:BBa_K1520507
OMPA-golB
OMPA-golB It is a protein to catch gold ions. OMPA present golB outside the membrane and golB will catch gold ions.
GolB is a identified small gold-specific protein in S. typhimurium that is highly induced in the presence of Au(I) over Cu(I), Ag(I), Zn(II), or Hg(II) ions. It has less than 70 amino acids in length and contains a similar metal binding motif, MTCXXC, to that of the previously reported human copper chaperone AtoX1 and yeast copper chaperone AtX1.
We display GolB on E. coli cell surface by fusing it with OmpA, an E. coli outer membrane protein to increase the disposing efficiency.
a) An illustration of the gold-specific protein GolB displayed on E. coli cell surface via the outer membrane protein OmpA.
b) SDS-PAGE and immunoblotting analyses of surface-displayed GolB protein.
1. uninduced bacteria are used as controls.
2. the supernatant protein fraction from induced bacteria.
3. bacteria membrane fraction from uninduced bacteria.
4. bacteria membrane fraction from induced bacteria expressing OmpA–GolB (The black arrow on the SDS-PAGE gel indicates the OmpA–GolB protein expressed)
c) Immunofluorescence labeling of E. coli cells using anti-FLAG antibody and FITC conjugated anti-mouse IgG antibody.
Metal selective adsorption of GolB displayed E. coli.(+)stands for containing OmpA-GolB plasmid, and (-) stands for not containing the plasmid. black stands for bacteria induced by 0.002% arabinose and the white ones were not induced by arabinose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 770
Illegal XhoI site found at 742 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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